Publikationen

 

 

Kretz J, Israel V, McIntosh M1, Design–Build–Test of Synthetic Promoters for Inducible Gene Regulation in Alphaproteobacteria, ACS Synthetic Biology, 2023, https://doi.org/10.1021/acssynbio.3c00251 (Genregulation)
 
 
Pantentantrag WO2023021108: Chromosomal integrating cassette allowing inducible gene expression for production of compounds via fermentation. Internationales Anmeldedatum: 17. August 2022, veröffentlicht am 23. Februar 2023, Antragsteller: Justus-Liebig-Universität Giessen, Erfinder:
Kretz J, Klug G, McIntosh M, World Intellectual Property Organization, https://patentscope2.wipo.int/search/en/detail.jsf?docId=WO2023021108&_gid=202308 (Genregulation)
 
 

Scheuer R,  Dietz T, Kretz J, Hadjeras L, McIntosh M,  Evguenieva-Hackenberg E, Incoherent dual regulation by a SAM-II riboswitch controlling translation at a distance, RNA Biology, Volume 19 –  Issue 1, 2022, https://www.tandfonline.com/doi/full/10.1080/15476286.2022.2110380 (Genregulation)

 

McIntosh M1, Köchling T, Latz A, Kretz J, Heinen S, Konzer A, Klug G, A major checkpoint for protein expression in Rhodobacter sphaeroides during heat stress response occurs at the level of translation,  Environ Microbiol. 2021 Oct 19. doi: 10.1111/1462-2920.15818 (Genregulation)

 

Gruetzner J, Remes B, Eisenhardt K, Scheller D, Kretz J, Madhugiri R, McIntosh M, Klug G, sRNA-mediated RNA processing regulates bacterial cell division. Nucleic Acids Research, 2021, 49(21):7035–7052 (Genregulation)

 

McIntosh M, Kretz J, Abbau von Braunalgen. Biospektrum (Heidelb), 2020, 26(6):646-651. doi: 10.1007/s12268-020-1461-8 (generell)

 

McIntosh M, Kretz J, Stickstofffixierung: Freundschaft oder Knechtschaft? Biospektrum (Heidelb), 2020, 26(6):646-651. doi: 10.1007/s12268-020-1461-8 (generell)

 

McIntosh M1, Eisenhardt K, Remes B, Konzer A, Klug G, Adaptation of the Alphaproteobacterium Rhodobacter sphaeroides to stationary phase. Environmental Microbiology, 2019, 21: 4425-4445 (Genregulation)

 

Ludueña LM, Anzuay MS, Angelini JG, McIntosh M, Becker A, Rupp O, Goesmann A, Blom J, Fabra A, Taurian T, Genome sequence of the endophytic strain Enterobacter sp. J49, a potential biofertilizer for peanut and maize. Genomics, 2019, 111: 913-920 (generell)

 

McIntosh M1, Serrania J, Lacanna E, A novel LuxR-type solo of Sinorhizobium meliloti, NurR, is regulated by the chromosome replication coordinator, DnaA, and activates quorum sensing. Molecular Microbiology, 2019, 112: 678-698 (Genregulation und Herstellung von Biopolymer)

 

Bathke J, Konzer A, Remes B, McIntosh M1, Klug G, Comparative analyses of the variation of the transcriptome and proteome of Rhodobacter sphaeroides throughout growth. BMC Genomics, 2019, 20: 358. doi: 10.1186/s12864-019-5749-3 (Genregulation)

 

Calatrava-Morales N, McIntosh M, Soto MJ1, Regulation Mediated by N-Acyl Homoserine Lactone Quorum Sensing Signals in the Rhizobium-Legume Symbiosis. Genes (Basel), 2018, 9:263. doi: 10.3390/genes9050263 (Genregulation)

 

Bettenworth V, McIntosh M, Becker A, Eckhardt B1, Front-propagation in bacterial inter-colony communication. Chaos, 2018, 28: 106316. Doi: 10.1063/1.5040068 (generell)

 

Ludueña LM, Anzuay MS, Angelini JG, McIntosh M, Becker A, Rupp O, Goesmann A, Blom J, Fabra A, Taurian T, Strain Serratia sp. S119: A potential biofertilizer for peanut and maize and a model bacterium to study phosphate solubilization mechanisms. Applied Soil Ecology 126, 2018, doi: 10.1016/j.apsoil.2017.12.024 (generell)

 

Baumgardt K, Melior H, Madhugiri R, Thalmann S, Schikora A, McIntosh M, Becker A, Evguenieva-Hackenberg E1, RNase E and RNase J are needed for S-adenosylmethionine homeostasis in Sinorhizobium meliloti. Microbiology (Reading), 2017, 163: 570-583 (Genregulation)

 

Ludueña LM, Anzuay MS, Magallanes-Noguera C, Tonelli ML, Ibañez FJ, Angelini JG, Fabra A, McIntosh M, Taurian T1, Effects of P limitation and molecules from peanut root exudates on pqqE gene expression and pqq promoter activity in the phosphate-solubilizing strain Serratia sp. S119. Res Microbiol, 2017, 168: 710-721 (generell)

 

Charoenpanich P, Soto MJ, Becker A, McIntosh M1, Quorum sensing restrains growth and is rapidly inactivated during domestication of Sinorhizobium meliloti. Environmental Microbiology Reports, 2015, 7: 373-382 (Genregulation und Herstellung von Biopolymer)

 

Schlüter JP, Czuppon P, Schauer O, Pfaffelhuber P, McIntosh M1, Becker A1, Classification of phenotypic subpopulations in isogenic bacterial cultures by triple promoter probing at single cell level. Journal of Biotechnology, 2015, 198: 3-14 (generell)

 

Vinardell JM, Acosta-Jurado S, Zehner S, Göttfert M, Becker A, Baena I, Blom J, Crespo-Rivas JC, Goesmann A, Jaenicke S, Krol E, McIntosh M, Margaret I, Pérez-Montaño F, Schneiker-Bekel S, Serranía J, Szczepanowski R, Buendía AM, Lloret J, Bonilla I, Pühler A, Ruiz-Sainz JE, Weidner S, The Sinorhizobium fredii HH103 Genome: A comparative analysis with S. fredii strains differing in their symbiotic behavior with soybean. Mol Plant Microbe Interact, 2015 28:811-24 (generell)

 

Baumgardt K, Charoenpanich P, McIntosh M, Schikora A, Stein E, Thalmann S, Kogel KH, Klug G, Becker A, Evguenieva-Hackenberg E1, RNase E affects the expression of the acyl-homoserine lactone synthase gene sinI in Sinorhizobium meliloti. Journal of Bacteriology, 2014, 196: 1435-47 (Genregulation)

 

McIntosh M, Czuppon P, Best K, Becker A, Pfaffelhuber P1, Modeling Quorum Sensing in Sinorhizobium meliloti. International Journal of Biomathematics and Biostatistics, 2013, 2:59-74 (Genregulation)

 

Carius L, Carius A, McIntosh M, Grammel H1, Quorum sensing influences growth and photosynthetic membrane production in high-cell-density cultivations of Rhodospirillum rubrum. BMC Microbiology, 2013, doi: 10.1186/1471-2180-13-189 (generell)

 

Charoenpanich P, Meyer S, Becker A1, McIntosh M, Temporal Expression Program of Quorum Sensing-Based Transcription Regulation in Sinorhizobium meliloti. Journal of Bacteriology, 2013, 195: 3224-3236 (Genregulation)

  

McIntosh M, Meyer S, Becker A1, Novel Sinorhizobium meliloti quorum sensing positive and negative regulatory feedback mechanisms respond to phosphate availability. Molecular Microbiology, 2009, 74: 1238-1256 (Genregulation)

 

Wollschläger K, Gaus K, Körnig A, Eckel R, Wilking S-D, McIntosh M, Majer Z, Becker A, Ros R, Anselmetti D, Sewald N, Single-Molecule Experiments to Elucidate the Minimal Requirement for DNA Recognition by Transcription Factor Epitopes. Small 2009, 5(4):484-95 (generell)

 

McIntosh M, Krol E, Becker A1, Competitive and Cooperative Effects in Quorum-Sensing-Regulated Galactoglucan Biosynthesis in Sinorhizobium meliloti. Journal of Bacteriology, 2008, 190: 5308-5317 (Genregulation und Herstellung von Biopolymer)

 

Bahlawane C, McIntosh M, Krol E, Becker A1, Sinorhizobium meliloti regulator MucR couples exopolysaccharide synthesis and motility. Mol Plant Microbe Interact, 2008, 21:1498-509 (Genregulation)

 

Anselmetti D, Bartels FW, Becker A, Decker B, Eckel R, McIntosh M, Mattay J, Plattner P, Ros R, Schäfer C, Sewald N1, Reverse engineering of an affinity-switchable molecular interaction characterized by atomic force microscopy single-molecule force spectroscopy. Langmuir, 2008, 24: 1365-70 (generell)

 

Bartels FW, McIntosh M, Fuhrmann A, Metzendorf C, Plattner P, Sewald N, Anselmetti D, Ros R, Becker A1, Effector-stimulated single molecule protein-DNA interactions of a quorum-sensing system in Sinorhizobium meliloti. Biophysics Journal, 2007, 92: 4391-400 (Genregulation)

 

McIntosh M, Stone B, Stanisich V, Curdlan and other bacterial (1‎→3)-beta-D-glucans. Applied Microbiology and Biotechnology, 2005, 68(2): 163-73 (Herstellung von Biopolymer)

 

Karnezis T, McIntosh M, Wardak AZ, Stanisich V, Stone B, The Biosynthesis of β-Glycans. Trends in Glycoscience and Glycotechnology, 2000, 12(66):211-227 (Herstellung von Biopolymer)

 

(1 gibt den korrespondierenden Autor an)

 

Zugehörige Publikationen:

Marjorie Hecht, German researchers find heat stress in Rhodobacter sphaeroides bacteria causes dramatic changes in RNA without affecting protein levels, Current Science Daily, May 11, 2022, https://currentsciencedaily.com/stories/623109764-german-researchers-find-heat-stress-in-rhodobacter-sphaeroides-bacteria-causes-dramatic-changes-in-rna-without-affecting-protein-levels

Patent:

Erst kürzlich wurde eine Erfindung von mir von der JLU Gießen beansprucht und wird für die Einreichung beim Patentamt vorbereitet. Die Erfindung des Patents ist ein gentechnisch verändertes Konstrukt, das das Chromosom so modifiziert, dass eine induzierbare Genexpression möglich ist. Das Problem, das dieses Patent adressiert, ist die Situation, dass eine Fülle von induzierbaren Genexpressionssystemen für Modellbakterien wie Escherichia coli und Bacillus subtilis existiert, diese aber bei der großen Mehrheit der weniger untersuchten Mikroben nicht oder nur schlecht funktionieren. Viele Nicht-Modellbakterien haben ein großes Potenzial für die Bioproduktion, könnten aber dennoch stark von einer genetischen Modifikation profitieren. Meine Erfindung wird einen Weg bieten, die Genexpression in fast jedem Bakterium von industriellem Interesse genetisch zu manipulieren.

Update August 2023: Das Patent wurde vom Europäischen Patentamt ( EPO ) angenommen und wird derzeit vom internationalen Patentamt (World Intellectual Property Organization) geprüft. 

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 matthew.mcintosh@mikro.bio.uni-giessen.de

 

Tel.: +49 (0)641 99-35557